Composite
Part:BBa_K1489006:Design
Designed by: Iowis Zhu Group: iGEM14_UMaryland (2014-10-16)
OmpA-CvGal1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
Illegal NheI site found at 1392 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 619
Illegal BamHI site found at 65 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1251
Design Notes
Gibson overhangs were designed for Gibson assembly
Source
CvGal1 is from the Crasseoterra virginica genome, codon optimized for E. coli expression.
OmpA is from the E. coli genome.