Composite

Part:BBa_K1489006:Design

Designed by: Iowis Zhu   Group: iGEM14_UMaryland   (2014-10-16)


OmpA-CvGal1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 1392
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 619
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1251


Design Notes

Gibson overhangs were designed for Gibson assembly


Source

CvGal1 is from the Crasseoterra virginica genome, codon optimized for E. coli expression.

OmpA is from the E. coli genome.

References